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Time domain analysis

You can create various maps with the following parameters.

  • Activation time
  • Rise time
  • Peak time
  • Peak amplitude
  • Repolarization time
  • Peak to repolarization time
  • APDxx
  • Decay τ
  • Upstroke velocity
  • Diastolic interval
  • Peak interval
  • Alternans
  • APDxx alternans

Select [Time domain analysis] from the [Analyze] menu.

On this dialog, a map can be created like below.

(1) Mask​

Select the map creation area from “All pixels”, “Mx (mask)”, “Rx (region)”, "Cx (circle)".

(2) Output type​

Select the type of map you want to create.

(3) Upstroke detection​

Select detection method for activation time from “Fmax/2”, “Fmax” and “max(dF/dt)”.

(4) Decay level (%)​

Set recovery % from the peak to baseline.

(5) Time range​

Set a time range. Peaks outside this time range are ignored.

(6) Min. interval​

Set a minimum time span between two peaks. This setting is used for automatic detection of peaks.

(7) Image display​

Shows the same data as the main screen.

The following mouse operations are possible on the image.

OperationDescription
Scroll mouse wheelEnlarge/reduce horizontal waveform size.
Mouse drag a pointLight intensity change at the specified pixel is displayed in (8) .


(8) Wave display​

Display light intensity change in the pixel specified in (7) .

The following mouse operations are possible on the waveform.

OperationDescription
Scroll mouse wheelEnlarge/reduce horizontal waveform size.
Mouse drag on waveform/Click on waveformMove frame.
Hold “Ctrl” key and drag mouse pointer to rightSelect time range of waveform.
Hold “Ctrl” key and drag mouse pointer to leftDeselect time range selection for waveform and select all ranges.

(9) CREATE MAPS​

Create one map for each peak in the selected time range.

Click this button to automatically detect peaks and display peak numbers at the top of each waveform.



(10) Display​

Select display mode from “Single”, “Average” and “Difference”.

  • Single : Display a map for each peak.
  • Average : Display average map of all peaks
  • Difference : Create a difference map between the two peaks

(11) Spike​

Specify number of peak (action potential or calcium transient) to display.

(12) Min​

Specifies minimum value to be displayed in color.

(13) Max​

Specifies maximum value to be displayed in color.

(14) Auto adjustment​

Click to set min and max to optimal values.

(15) Plot options​

Click to open plot options. Color map (palette) can be changed.

(16) Ref. point​

Set the point where value=0 so that all data can be mapped to the same color scale. This has no effect for time span values (e.g. APD).

(17) X​

Displays the X coordinate of the reference point. You can change this value.

(18) Y​

Displays the Y coordinate of the reference point. You can change this value.

(19) Min​

Set the “ (17) Ref. point” position to the min. value position.

(20) Annotations: Min​

Annotate minimum value points.

(21) Annotations: Max​

Annotate maximum value points.



(22) Title and spike​

The image title and spike # are automatically displayed.

(23) Map​

A map created for each peak is shown here.

The following mouse operations are possible.

OperationDescription
Scroll mouse wheelEnlarge/reduce horizontal waveform size.
Mouse move over imageCoordinates and values are displayed on the top left of the map.

(24) Ref. point​

Select “ (16) Ref.point” to display the point on the image. Mouse drag the point where value=0 so that all data can be mapped to the same color scale.



(25) Distribution​

Displays a histogram showing the relationship between values and number of pixels. Scrolling mouse wheel enlarges/reduces size of histogram.

(26) Mean vs time​

Displays mean values for each peak.



(27) GRID VIEW​

Display multiple created maps in a grid. The number of columns can be changed with "Columns".

(28) SAVE VALUES​

Save data to a binary file (*.dat).

(29) SAVE FIGURE​

Export map as an image file. Click to open “Figure editor”. See “Save image (figure editor)” for details.

(30) SAVE STATISTICS​

Save numerical values of displayed map to a CSV file.

(31) CLOSE​

Close this dialog.