MiCAM ULTIMA, is a CMOS based ultra high speed imaging system that optimizes high speeds, high resolution, and high signal to noise ratios. It is designed to detect very small changes in fluorescent light levels of biological samples that are stained with fluorescence probes such as voltage-sensitive dyes or calcium indicators.
MiCAM ULTIMA
* MiCAM ULTIMA was discontinued. Next model MiCAM03-N256 is now available.
* For
software download, go to
the "Download" page.
Main Features
- Spatial Resolution
- 100 x 100 pixels
- Image Sensor Size
- 10mm x 10mm
- Camera Port
- 2
- Maximum Frame Rate
- 10,000fps (100x100 pixels)
Applications
- Membrane potential (voltage sensitive dye) imaging
- Calcium imaging for in vivo brain/cardiomyocytes
- Imaging with FRET, GCaMP, GEVI
- Intrinsic optical signal imaging based on hemoglobin and flavoprotein autofluorescence
- Ratiometric fluorescence imaging with 2 camera heads
- Panoramic imaging with 2 camera heads
- Other high speed imaging
Features
Maximum Frame Rate of 0.1msec/frame (10KHz)
In order to record small changes in fluorescence of biological samples stained with voltage
sensitive dyes, the ideal combination of high sensitivity and high speed is required. MiCAM
ULTIMA, which overcomes these challenges with BrainVision's cutting-edge research and
development, can detect small changes in fluorescence such as 0.1% After acquisitions, users can
easily analyze signal propagation between neurons of samples such as hippocampus slices at
speeds up to 10,000 frames per second and only 4 times averaging.
Real-Time Imaging of Action Potential Propagation with Single Scan
Temporal resolution of 0.1msec/frame, spatial resolution of 100x100pixel, and dynamic range of
70db makes it possible for scientists to record neuronal activity at speeds of several
millisecond/frame even without any averaging acquisition (single scan).
Huge Well Depth of Single Pixel (10Me- / 1.5Me-)
Sufficient fluorescence light intensity is needed to detect small changes in fluorescence with a
single sweep. MiCAM ULTIMA, which has a huge well depth (10Me- / 1.5Me-), can achieve high S/N
ratios without any pixel saturation in most bright light conditions.
Completely Synchronized Dual Camera System
MiCAM ULTIMA processor has two camera ports. The ULTIMA can operate as a synchronized dual camera system by the simple addition of a slave camera and connection to the existing processor. This allows for dual-wavelength imaging or dual-staining with multiple probes such as voltage-sensitive dyes and calcium indicators.
Software with Intuitive Interface
BV_Analyzer is a data analysis software and is included with all of the MiCAM systems. This
software has various functions, such as wave display, 3D display, animation display, real-image
display, spatial/temporal filter, high-pass/low-pass filter, AVI/TIFF/BMP/CSV file export, etc.
Characteristics of Wave Spectral Sensitivity
This sensor has a wide range of spectral sensitivity between near-infra red (800-900nm) to
ultraviolet (300-400nm), and has a high quantum efficiency of 50-60%. As a result, the ULTIMA is
suitable for various imaging techniques with many different kinds of fluorescent dyes.
Specifications
| Model | MiCAM ULTIMA | |
|---|---|---|
| ULTIMA-H | ULTIMA-L | |
| Sensor | Original CMOS | |
| Actual Sensor Size | 10mm x 10mm | |
| A/D Converter | 14bit (Data resolution is 16 bit after digital process) | |
| Spatial Resolution (pixels) | 100(H) x 100(V) | |
| Temporal Resolution (/frame) | 0.1-250.0ms | |
| Maximum Frame Number | 20,480 (Option: 40,000) | |
| Well-Depth | 10,000,000e (Option: 24,000,000e) | 1,500,000e (Option: 3,000,000e) |
| Actual Quantum Efficiency | 63%@550nm | |
| 45%@700nm | ||
| Dark Noise | 920e@0.1msec | 230e@0.1msec |
| 400e@1.0msec | 80e@1.0msec | |
| Included | Processor, Cameras, Computer, Monitor, Acquisition/Analysis software | |
| Output/Input | 2 analog inputs (6 additional channels available), 8 digital outputs, 8 digital inputs, timing output, programmable pulse sequencer output, live display monitor output, synchro output | |
| Acquisition Software | Trigger signal output, Focus monitoring, Pre-trigger recording, Multi-stimulus output, Frame rate setting, Frame number setting, Real-image display, Master/Slave mode, Shutter controlling pulse, Auto-reference function, Auto background image recording and so on | |
| Analysis Software | dF/F, Averaging, Addition, Division, Spatial filter, Cubic filter, Temporal filter, Bleaching compensation, Vibration compensation, High-pass/Low-pass filter, Reference frame setting, 3D display, Wave display, ROI wave display, Movie, Multi image display, Real image display, AVI/TIFF/BMP/CSV output and so on | |
Next Generation of MiCAM ULTIMA
- High Speed Imaging System
- MiCAM03-N256
MiCAM03-N256 is a high-speed imaging system that captures and visualizes small changes in fluorescence intensity from biological samples stained with fluorescent probes, such as voltage sensitive dyes and calcium dyes.
- Spatial Resolution :
- 128x128 - 256x256 pixles
(32x32 pixels - 256x256 pixles with option) - Maximum Frame Rate :
- 1,000fps
(20,000 fps available with option) - Up to 2 camera heads can be used with completely synchronization.