MiCAM02-CMOS

* MiCAM02 system was discontinued. Next model MiCAM03-N256 is now available.
* Camera head for MiCAM02 (02-HR, CMOS) is still available.

MiCAM02-CMOS is a CMOS camera that is compatible with the MiCAM02 imaging system. The originally designed CMOS sensor achieves higher frame rates and a wider dynamic range than the current CCD models. The new CMOS camera provides high resolution movies at the sub-millisecond level and creates significant advantages in high speed imaging applications.

Main Features

Spatial Resolution
92 x 80 pixels / 188 x 160 pixels: Image Sensor Size
5.76mm x 4.8mm: Maximum Frame Rate
1,666fps (92 x 80 pixels) 833fps (188x160 pixels)

Applications

Membrane potential (voltage sensitive dye) imaging
Calcium imaging for in vivo brain/cardiomyocytes
Imaging with FRET, GCaMP, GEVI
Intrinsic optical signal imaging based on hemoglobin and flavoprotein autofluorescence
Ratiometric fluorescence imaging with 2 camera heads
Panoramic imaging with 2 camera heads
Other high speed imaging

Features

Original CMOS image sensor developed by Brainvision Inc.
Higher frame rate (max: 0.6msec/frame) and lower noise

CMOS Image Sensor
MiCAM02-CMOS sensor The original CMOS image sensor adapted for the MiCAM02-CMOS camera is designed by Brainvision Inc. To achieve signal readout with higher speed and lower noise, many parts from pixel to readout circuit are tuned up with the latest CMOS technology. This allows the CMOS camera to record at higher frame rates of up to 0.6msec/frame at 92x80 pixels, and 1.2msec/frame at 188x160 pixels.

Wide dynamic range over 68dB

The highly customized CMOS sensor has a well depth of 450,000e- and wide dynamic range of 68dB or more. With sufficient light intensity emitted from a biological sample, it is possible to achieve higher S/N ratios than MiCAM02-HR and MiCAM02-HS.

Compatible with MiCAM02

Current MiCAM02 users can easily utilize the new MiCAM02-CMOS camera by simply connecting the camera to the processor and updating the software version.

Synchronized dual-camera system for dual-wave length imaging

Two CMOS cameras can be connected to the MiCAM02 processor to do synchronized recordings. This dual-camera system can be used to simultaneously image voltage-sensitive dye and calcium ion indicators, as well as perform 3D mapping of multiple locations on a biological sample.

Sample Data

Imaging of Action Potential Propagation in Isolated Rat Heart

An action potential propagation in isolated rat heart. The heart was Langendorff perfused, stained with Di-4-ANEPSS, and recorded at 1,000 frames/second and 92x80 pixels using MiCAM02-CMOS camera.

Specifications

Process Technology	0.18CIS-P1M6
Resolution (pixels)	188(H) x 160(V) / 92(H) x 80(V)
Pixel Size	30 μm square
Pixel Aperture Ratio	63%
Well Depth	450,000 e-
Readout Noise	< 150e-
Dynamic Range	> 68dB
Temporal Noise Factor	< -57dB
Temporal Noise Factor	< -57dB
Chip Size	7.0mm square
Max. Frame Rate	1,700 fps
Compatibility	MiCAM02
Shutter	Original Global Shutter

Next Generation of MiCAM02

MiCAM03-N256 is a high-speed imaging system that captures and visualizes small changes in fluorescence intensity from biological samples stained with fluorescent probes, such as voltage sensitive dyes and calcium dyes.

Spatial Resolution :: 128x128 - 256x256 pixles
(32x32 pixels - 256x256 pixles with option)
Maximum Frame Rate :: 1,000fps
(20,000 fps available with option)
Up to 2 camera heads can be used with completely synchronization.