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  • MiCAM03-N256
MiCAM03-N256

High-Speed Optical Mapping System MiCAM03-N256

MiCAM03-N256 is a high-speed optical mapping system that captures and visualizes small changes in fluorescence intensity from biological samples stained with fluorescent probes, such as voltage sensitive dyes and calcium dyes.

This product is for research use only (RUO).

High-Speed Optical Mapping System MiCAM03-N256

Applications

  • Membrane potential (voltage sensitive dye) imaging
  • Calcium imaging for in vivo brain/cardiomyocytes
  • Imaging with FRET, GCaMP, GEVI
  • Intrinsic optical signal imaging based on hemoglobin and flavoprotein autofluorescence
  • Ratiometric fluorescence imaging with 2 camera heads
  • Panoramic imaging with 2 camera heads
  • Other high speed imaging

Main Features

Spatial Resolution
256 x 256 pixles, 128 x 128 pixels
(Optional license allows to select more pixel settings)
Image Sensor Size
17.6mm x 17.6mm
S/N Ratio
> 71dB
Camera Port
2
Memory
1GB
Maximum Frame Rate
1,000fps (256 x 256px),  1,000fps (128 x 128px)
(Optional license allows to select faster frame rates)

Sample Data

Isolated Pig Heart 1,818fps at 256x256 pixels

Sample
Isolated pig heart
Fluorescence Dye
Voltage sensitive dye
Mapping System
MiCAM03-N256
Pixels
256x256
Frame Rate
1,818fps (0.55msec/frame)
Provided by
Dr. Jack M. Rogers, The University of Alabama at Birmingham

Monolayer of neonatal mouse ventricular myocyte stained with voltage sensitive dye

Activation Map
Sample
Monolayer of neonatal mouse ventricular myocyte. Sample was scratched with a needle tip at three locations (visible on the fluorescence pictures) to induce conduction blocks and meandering of the wavefront.
Method
Sample is paced locally at different frequencies using a glass microelectrode positioned at the edge of the sample
Fluorescence Dye
Voltage sensitive dye (Di-8-Anepps)
Mapping System
MiCAM03-N256
Pixels
256x256
Frame Rate
1,000fps (1.0msec/frame)
Provided by
Dr. Jan Kucera and Dr. Ange Maguy, Department of Physiology, University of Bern

Features : Camera Head

N256 camera head front N256 camera head rear

N256 camera head is equipped with a new CMOS image sensor designed and developed by Brainvision Inc.

Large Sensor and Large Pixel

Active Area size
17.6mm x 17.6mm
Pixel size
69μm x 69μm

CMOS image sensor Applications such as membrane potential imaging require both very high speed, and the ability to detect small changes in fluorescence, such as 0.5-1%. In order to optimize S/N ratios, it is important to increase the amount of light captured by one pixel. It is easy to collect light and capture small changes using a sensor having large pixels.

High Spatial Resolution, High Frame Rates, and High S/N Ratios

Number of Pixels
256 x 256 pixels
Maximum Frame Rate
1,000 fps (at 256x256 pixels)
Dynamic Range
71dB

The overall performance has been improved in a balanced manner. This includes maximum frame rate, spatial resolution, and S/N ratios, all of which are important factors for high-speed membrane potential imaging.

Wide Dynamic Range Mode or High Sensitivity Mode by Adjustable Well Depth

Wide Dynamic Range Mode
3,000,000 electrons
High Sensitivity Mode
   600,000 electrons

It is now possible to easily switch the full well capacity of the camera in the acquisition software. Two modes are available: wide dynamic range mode (D-mode), and high sensitivity mode (S-mode).

D-mode is used for tissue samples with high fluorescence light intensity, and S-mode is used for cultured cell monolayers, or preps with low fluorescence light intensity. This adjustable well depth makes it possible to image a variety of biological samples using a single camera.

The well-depth is fixed regardless of settings such as sampling rate.

ROI Readout for Higher Frame Rates (Option)

Preset partial readout setting and multiple ROI setting can be selected, and as a result it is possible to achieve higher frame rates. For example, ultra high frame rates such 20,000 fps@32x32 pixels is possible.

  • All pixels readout (256x256 pixels) All pixels readout (256x256 pixels)
  • Multiple ROI readout (32x32x9 pixels) Multiple ROI readout (32x32x9 pixels)

Features : Processor

MiCAM03 processor

Direct Data Saving and Long-Term Data Acquisition with USB3.0 High-Speed Data Transfer

New USB3.0 interface allows for significantly faster data transfer from processor to PC. It is now possible to save data directly to HDD or SSD, and long-term recording of several minutes up to a few hours can be achieved, regardless of the RAM capacity (Note that sampling rate, number of pixels, number of cameras used, and PC specifications, will affect the total recording time).

Approximate number of recordable frames and recordable time
Disk Capacity Pixels Frame Rate (fps) Number of recordable frames Recordable time (min)
SSD 1TB 256x256 500 6,840,000 228.0
1,000 6,840,000 114.0
1,818 6,840,000 62.7
128x128 500 27,360,000 912.0
1,000 27,360,000 456.0
5,263 27,360,000 86.6

(*Approximate number of recordable frames and recordable time shown above are averaged values that were measured using the PC configuration which is included with MiCAM03. There is no guarantee that the results will be the same in all circumstances.)

Up to two cameras can easily be connected and used in a completely synchronized dual-camera system

MiCAM03 processor has two camera ports, and can be adapted as a dual-camera system using an optional second camera, which enables synchronized recordings. This allows for simultaneous imaging of multiple fluorescent wavelengths, as well as three-dimensional imaging from different angles.

  • Camera port of MiCAM03 processor

Synchronous Lighting Control of LED Light Source for Two-Wavelength Excitation Imaging such as GCaMP Imaging

In the case of imaging using two fluorescent probes with two different excitation wavelengths or imaging using a two-wavelength excitation/single-wavelength fluorescent probe, it is necessary to synchronize the frame timing of the camera and the lighting timing of the light source.

This function is to output the lighting signal synchronized with each frame timing from MiCAM03 to multiple LED light sources. This makes it possible to easily synchronize the frame acquisition with MiCAM03 and LED lighting.

There are up to 4 channels of output. Preset patterns, in which signals are output alternately from each channel, are available. There is no need to set the lighting pattern using a commercially available pulse generator.

Moreover, since the pulse delay time and pulse width can be specified by software, it is possible to make detailed settings in accordance with the rise and fall times of the LED used.

  • Two-wavelength excitation mode Two-wavelength excitation mode

Two-wavelength excitation for GCaMP imaging using LEX9 and MiCAM

Various I/O Connectors, and Improved Compatibility with External Devices

MiCAM03 has multiple input and output connections available. These universally standard I/O connections can be synchronized with image acquisition and external devices.

  • Front Panel

Other Features

Turnkey System Ready for Immediate Use

The system consists of hardware/software specifically designed for high-speed fluorescence imaging, and the PC and monitor are included as standard equipment. Before use, calibration/setting/tuning is not necessary. The hardware connection is straightforward between components using cables, making it possible to acquire data immediately after setup.

  • Diagram of MiCAM system

Pre-Post Trigger Mode

MiCAM03 can record activity which occurs before and after a trigger signal is generated. When an external trigger occurs, such as a spontaneous biological signal, or a trigger pulse from an external stimulator, a specified amount of "pre-trigger" data can be saved. The "post-trigger" data is also collected in the same recording. As a result, both pre-trigger data and post-trigger data can be saved within one acquisition period.

  • Pre-Post Trigger Mode Pre-Post Trigger Mode (75% Pre-Trigger)

Time Lapse Imaging

MiCAM03 can capture phenomenon that changes in a few minutes up to several hours, intermittently. Because illumination light is turned on only at the time of image acquisition, phototoxic effects to sample is minimized, and a stable baseline can be acquired.

  • Example of Time-lapse imaging Example of Time-lapse imaging

High Resolution Camera Control Function (Optional)

It is possible to control a high resolution camera having 1920x1200 pixels (BV-XB1 camera). High resolution imaging can be applied to various applications, including detection of intracellular Ca 2+ dynamics, flavoprotein fluorescence, FRET imaging, and also imaging of animal behaviors. High resolution imaging with BV-XB1 can also be synchronized with MiCAM03 high-speed imaging.

  • BV-XB1 camera BV-XB1
  • Example of flavoprotein imaging Example of flavoprotein imaging

Software

Acquisition/Analysis Software (BV Workbench)

BV Workbench is the image acquisition software that controls MiCAM03-N256 optical mapping system. In addition to image acquisition and control of inputs and outputs of the optical mapping system, analysis functions including movie playback, wave display, filter functions, etc…are also included. BV Workbench has an intuitive user interface that is easily operated with the use of a mouse.

Acquisition/Analysis Software BV Workbench

Cardiac Optical Mapping Data Anaysis using BV Workbench Version 4.5.1

Specifications

System
Name High-Speed Optical Mapping System
MiCAM03-N256
High Speed Optical Mapping System
MiCAM03-HR
Model MC03-N256 MC03-HR
Standard System Configuration Camera Head (1-2), Processor, PC, Monitor,
Acquisition software, Analysis Software
Supported OS Windows 7/10 64bit
Camera Head
Name N256 camera HR camera
Model MC-N256CAM 02HRCM
Image Sensor Original CMOS Sony CCD
Active Pixels (H x V) 256 x 256 380 x 244
Active Image Area (H x V) 17.6mm x 17.6mm 6.4mm x 4.8mm
Pixels Size (H x V) 69μm x 69μm 16.8μm x 19.6μm
Shutter Mode Global Global
Maximum Frame Rate and
Pixels (for 1 camera)
1,923fps (256x256px)
3,125fps (192x192px)
5,556fps (128x128px)
20,000fps (32x32px)
143fps (380x244px)
270fps (188x120px)
455fps (92x60px)
Quantum Efficiency 55%@550nm / 50%@600nm 75%@550nm / 50%@700nm
Well-Depth 3,000,000e- / 600,000e- (switching) 100,000e- (180x120px) /
12,000e- (180x120px, H-bin)
Dark Noise 500e- / 130e- 60e- (3.7ms,180x120px) /
20e- (3.7ms,180x120px,H-bin)
Lens mount Original M42 mount
(flange back:14.5mm)
(Option: C or F mount)
C mount
Interface Special Cable Special Cable
Dimension (WxHxD) 80mm x 80mm x 50mm 62mm x 52mm x 46mm
Weight 474g 100g
Processor
Name MiCAM03 processor MiCAM03 processor (analog I/F)
Model MC03-PRC MC03-PRC-A
Camera Interface 1
Camera Port 2
Slot for Optional Unit -
Buffer Memory Inside Processor 1GB
Analog Inputs for
Synchronous Recording
2ch
Output for Stimulation Pulse 1ch
Input for External Trigger Signal 1ch
Output for Lighting Control 1ch
Input/Output for Frame Timing 1ch
Output for Real Time
Light Intensity Monitor
-
Output for Light Intensity Control -
Current Output -
Output for Acquisition Status -
Output for Trigger Wait Status -
Digital I/O port 1 (6 pins)
Trigger Mode Software / TTL External Input / Analog Input / Usage of Optional Unit
Data Transfer Mode USB3.0
Recording Mode Temporary storage to PC RAM / Direct data saving to PC drive (SSD/HDD)
Maximum Recordable Frame Number Dependent on camera used and image acquisition settings
Power Supply AC100V-230V、50Hz/60Hz 150W
Fuse T2A L 250V
Dimension 300mm (W) x 260mm (D) x 60mm (H)
Weight 2.6kg
Default/Optional Function
Single Trial Acquisition Default
Long Time Acquisition
(direct saving to disk)
Option (sold separately)
Averaging Acquisition Default
Multiple ROI Readout for
Higher Frame Rate
Option (sold separately)
Time-Lapse Acquisition Option (sold separately)
High Resolution Camera Control Option (sold separately)
Acquisition software Default
Analysis software Option (sold separately)
SDK (for Visual C# .NET and MatLab) Option (sold separately)
* Specifications and appearance are subject to change without prior notice due to continuous improvements.
* This product is for research use only. * This product is made in Japan.
Selectable Number of Pixels and Maximum Frame Rate (N256 Single Camera)
Selectable Number of Pixels (HxV) Maximum Frame Rate (fps)
NR Mode OFF NR Mode ON
2 Times Averaging
NR Mode ON
4 Times Averaging
256x256 Full Resolution 1,818 1,923 1,031
192x192 Square ROI 2,941 3,125 1,695
128x128 5,263 5,556 3,333
64x64 12,500 12,500 8,333
256x128 Horizontally Long
Rectangle ROI
3,333 3,448 1,887
256x64 5,882 5,555 3,226
256x32 9,091 8,333 5,000
128x256 Vertically Long
Rectangle ROI
3,030 3,333 1,852
64x256 4,348 5,000 3,125
32x256 5,556 7,143 4,762
32x32 16 blocks 2,381 2,632 1,724
15 blocks 2,500 2,778 1,818
14 blocks 2,703 2,941 1,961
13 blocks 2,857 3,125 2,083
12 blocks 3,125 3,448 2,273
11 blocks 3,333 3,704 2,439
10 blocks 3,704 4,000 2,703
9 blocks 4,000 4,348 2,941
8 blocks 4,545 5,000 3,226
7 blocks 5,000 5,556 3,704
6 blocks 5,556 6,250 4,167
5 blocks 6,667 7,143 5,000
4 blocks 7,692 8,333 5,882
3 blocks 10,000 11,111 7,692
2 blocks 12,500 14,286 10,000
1 blocks 20,000 20,000 16,667
Selectable Number of Pixels and Maximum Frame Rate (N256 Dual Camera)
Selectable Number of Pixels (HxV) Maximum Frame Rate (fps)
NR Mode OFF NR Mode ON
2 Times Averaging
NR Mode ON
4 Times Averaging
256x256 Full Resolution 1,087 1,064 1,031
192x192 Square ROI 1,818 1,754 1,695
128x128 3,704 3,571 3,333
64x64 9,091 8,333 8,333
256x128 Horizontally Long
Rectangle ROI
2,041 1,961 1,887
256x64 3,571 3,226 3,125
256x32 5,882 5,000 5,000
128x256 Vertically Long
Rectangle ROI
2,000 1,961 1,852
64x256 3,571 3,448 3,125
32x256 5,556 5,556 4,762
32x32 16 blocks 1,887 1,613 1,613
15 blocks 2,041 1,724 1,724
14 blocks 2,174 1,852 1,852
13 blocks 2,326 1,961 1,961
12 blocks 2,500 2,128 2,128
11 blocks 2,703 2,326 2,326
10 blocks 2,941 2,500 2,500
9 blocks 3,226 2,778 2,778
8 blocks 3,571 3,125 3,125
7 blocks 4,000 3,448 3,448
6 blocks 4,762 4,000 4,000
5 blocks 5,556 4,762 4,762
4 blocks 6,667 5,556 5,556
3 blocks 8,333 7,143 7,143
2 blocks 11,111 10,000 10,000
1 blocks 16,667 16,667 16,667

Brochure Download

  • Brochure MiCAM Series

    High-Speed Optical Mapping System MiCAM Series (2019)

  • Brochure MiCAM05-N256 and MiCAM03-N256

    MiCAM05-N256 and MiCAM03-N256

Products
  • Optical Mapping System
    • MiCAM05-N256
    • MiCAM03-N256
    • MiCAM03-C35IR
    • BV-XB1
  • Software
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  • Light Source
  • Multi-Device Synchronizer
  • Cardiac Signal Acquisition
  • Others
  • Legacy Products
MiCAM03-N256
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  • Sample data
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  • Brochure download

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