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High Resolution Imaging System for GCaMP Imaging
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BV-XB1
BV-XB1 is a high resolution imaging system which combines a high
resolution camera with 1920 x 1200 pixels, plus image acquisition
software developed for bioimaging experiments.
The system
includes ESTM-9, which is equipped with external camera control,
external illumination control, pulse outputs, analog outputs, and
analog inputs recording functionality.
This product is for research use only (RUO).
Main Features
- High Resolution
- 1920x1200 pixels
- Large Sensor
- 11.3mmx7.1mm
- Wide Field of View
- 7.1mmx4.4mm -
17.9mm x 11.2mm (with THT)
- Wide Dynamic Range
- 73.7dB
- Low Dark Noise
- 6.6e-
Applications
- Widefield calcium imaging
- Intrinsic optical signal (IOS) imaging
- GCaMP imaging
- FRET imaging
Features : XB1 Camera Head
The dark noise of the CMOS sensor used in the XB1CM camera head is as low as
that of CCD sensors, and it is possible to capture images at relatively high
frame rates. Due to this combination of low noise and relatively high speed,
XB1CM is suitable for low light level bioimaging applications, such as
calcium imaging, imaging using fluorescent proteins such as GCaMP/FRET, and
flavoprotein intrinsic imaging.
High Resolution, Large Sensor and Wide Field of View
Spatial resolution: 1920x1200 pixels, sensor size:11.3mmx7.1mm
| Total Magnification of Macroscope |
Field of View |
| 0.63x |
17.9mm x 11.2mm |
| 1.0x |
11.3mm x 7.1mm |
| 1.6x |
7.1mm x 4.4mm |
Maximum frame rate and sensitivity can be changed by number of pixels
and binning
| Binning |
Resolution |
Maximum frame rate (fps) |
Frame interval
(ms)
|
| 1x1 |
1920x1200 |
Full pixel readout |
78.11 |
12.8 |
| 960x600 |
Partial readout |
249.44 |
4.0 |
| 640x400 |
362.84 |
2.8 |
| 480x300 |
469.48 |
2.1 |
| 384x240 |
570.13 |
1.7 |
| 2x2 |
960x600 |
Full pixel readout |
128.77 |
7.8 |
| 480x300 |
Partial readout |
249.44 |
4.0 |
| 240x150 |
469.48 |
2.1 |
| 3x3 |
640x400 |
Full pixel readout |
128.77 |
7.8 |
| 320x200 |
Partial readout |
249.44 |
4.0 |
| 160x100 |
469.48 |
2.1 |
| 4x4 |
480x300 |
Full pixel readout |
128.77 |
7.8 |
| 240x150 |
Partial readout |
249.44 |
4.0 |
| 192x100 |
307.03 |
3.3 |
| 160x100 |
362.84 |
2.8 |
(*1) Size of effective pixel will change, dependent on binning.
(*2) Size of effective sensor area and size of effective pixel will
change, depending on selected number of pixels and binning.
Features : ESTM-9
By connecting external equipment such as camera, light source, electrical
stimulator, and amplifiers, ESTM-9 can synchronize image acquisition with
inputs/outputs of external equipment.
Acquisition Control of External Camera
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Outputs frame timing pulses to external camera, or receives frame
timing signals from external camera.
- Time-lapse acquisition control is also available.
Light Source Control
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Outputs signals to external light source in synchronization with
camera control.
- Continuous, intermittent, or custom light pattern available.
Pulse Stimulator
- 4 channels of pulse stimulation can be controlled individually.
- Stimulation works with or without camera control.
- Continuous stimulation using free-run mode is also available.
Analog Signal Recording
- 2 channels of analog inputs.
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Pre-trigger, delayed-trigger, and multiple trigger source available.
Other Features
Turnkey System Ready for Immediate Use
The system consists of hardware/software specifically designed for
high-speed fluorescence imaging, and the PC and monitor are included as
standard equipment. Before use, calibration/setting/tuning is not
necessary. The hardware connection is straightforward between components
using cables, making it possible to acquire data immediately after
setup.
Multi Camera System
By connecting multiple XB1CMs to PC, it is possible to operate as a
fully synchronized multi camera system. Two images can be superimposed
on monitor display so that user can overlap two images.
Time Lapse Imaging
BV-XB1 can capture phenomenon that changes in a few minutes up to
several hours, intermittently. Because illumination light is turned on
only at the time of image acquisition, phototoxic effects to sample are
minimized, and a stable baseline can be acquired.
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Example of Time-lapse imaging
Software
Acquisition/Analysis Software (BV Workbench)
BV Workbench is the image acquisition software that controls
MiCAM03-C35IR optical mapping system. In addition to image acquisition
and control of inputs and outputs of the imaging system, analysis
functions including movie playback, wave display, filter functions,
etc…are also included. BV Workbench has an intuitive user interface that
is easily operated with the use of a mouse.
Specifications
| System |
| Name |
High Resolution Imaging System BV-XB1 |
| Model |
BV-XB1 |
| Standard System Configuration |
Camera Head (1), ESTM-9, PC, Monitor,
Acquisition software,
Analysis Software
|
| Supported OS |
Windows 10 64bit |
| Camera Head |
| Name |
XB1 Camera Head |
| Model |
XB1CM |
| Image Sensor |
SONY IMX174 CMOS (1/1.2inch) |
| Resolution (H x V) |
1920 x 1200 |
| Active Image Area (H x V) |
11.3mm x 7.1mm |
| Pixel Size (H x V) |
5.86um x 5.86um |
| Shutter Mode |
Global Shutter |
| Maximum Frame Rate and Pixels |
78.11fps@1920x1200pixels
249.44fps@960x600pixels
362.82fps@640x400pixels
469.48fps@480x300pixels
570.13fps@384x240pixels
|
| Quantum Efficiency |
70% |
| Dark Noise |
6.6e- |
| Saturation Capacity |
31.7ke- |
| Dynamic Range |
73.7dB |
| Pixel Bit Depth |
12bit |
| Interface |
USB3.0 |
| Lens Mount |
C-mount |
| Dimension |
29.3mm x 29mm x 29mm |
| Weight |
80g |
| ESTM-9 |
| Pulse Output |
4 ch (BNC) |
| Analog Input |
2 ch (BNC) |
| Analog Output |
2 ch (BNC) |
| Camera I/O Port for XB1CM |
2 ch (6 pin connector) |
| Frame Timing Signal Output |
1 ch (BNC) |
| Acquisition Status Output |
1 ch (BNC) |
| Shutter Control Output |
1 ch (BNC) |
| External Trigger Input |
1 ch (BNC) |
| Others |
Trigger button, Stop button,
8-pin connector for optional
function
|
| Interface |
USB2.0 |
| Power |
USB bus power |
| Dimensions |
180mm x 120mm x 65mm |
| Each function |
| Main Trigger |
Trigger Mode |
Software / External / Internal |
| Trigger Source |
Software / External Trigger Input / Trigger Button |
| Trigger Edge |
Positive / Negative |
| Pulse Output |
Output Voltage |
5V |
| Clock Cycle |
10μsec - 10msec |
| Duration time |
10μsec - 10min |
| Delay Time |
10μsec - 10min |
| Interval Time |
10μsec - 10min |
| Pulse Number |
1 - 65,535 |
| Analog Input |
Input Range |
1V / 3V / 10V |
| Sample Clock |
10μsec - 10msec |
| Trigger Source |
Main / Pulse1 / Pulse2 / Pulse3 / Pulse4 / Ain1 level |
| Others |
Previous-trigger, Delayed-trigger, Low-pass filter, Polarity
inversion
|
| Analog Output |
Output Voltage |
-10V - 10V |
| Output Waveform |
Sine, Triangle, Sawtooth, Custom (with a CSV file) |
| Output Waveform |
Number of Camera Port |
Brainvision BV-XB1 : 2 (I/O connectors)
Other camera : 1 (BNC connectors)
|
| Frame Timing Signal |
Output or Input |
| Frame Timing Signal Cycle |
10μsec-300msec |
| Trigger Source |
Main/Ain1 level/Pulse3 |
| Others |
Time lapse |
| Light Control |
Lighting Mode |
Continuous Synchronous with data acquisition /
Synchronous
with frame acquisition / Custom lighting pattern
|
| Others |
Polarity inversion |
* Specifications and appearance are subject to change without prior
notice due to continuous improvements.
* This product is for research use only.
* This product is made in Japan.
