Dynamic range optimization (DRO)

This filter is automatically applied when applying various filters without being specified by user.

A dark image may be captured depending on staining condition of sample, problems with optical system, or sampling rate conditions. In this case, only a part of the 16-bit gradation is used.

With Dynamic range optimization, brightness value of each pixel is optimized to use entire 16-bit gradation, and dark image is corrected to be bright.

The algorithm is as follows.

1. Detect the minimum and maximum values of all pixels in all frames.
2. Calculate gain and offset so that the minimum value of 1 is 0 and the maximum value is 65535.
3. Apply gain of 2 and offset to all frames and all pixels.