Basic System for Optical Mapping BV-XB2

1. Start Experiments Right After Installation, with No Setup Hassle — All Necessary Equipment in One Set

Because all the equipment needed for experiments is bundled into a single package, there is no need to select and procure peripheral devices individually. This greatly reduces setup time and provides an environment where you can focus on the research itself.

2. Start Optical Mapping Easily on a Limited Budget — Lower Price Than Before

By adopting a general-purpose high-speed camera and simple optics, we have achieved a significantly lower price than our previous systems.

While the S/N ratio is lower than that of higher-end models, the analysis software, LED light source, and electrical stimulator are shared with the higher models, so you can conduct research with a professional-grade workflow at an affordable price.

3. Record Action Potential Propagation in Detail — High-Speed Imaging at Over 1,000 Frames/Second

To accurately capture action potentials that change on a millisecond scale, a speed of 1,000 fps (1 ms/frame) or higher is essential.

This system enables high temporal resolution through ROI (Region of Interest) partial readout. It depicts neural and cardiac propagation patterns in detail, enabling highly reliable data acquisition.

Frame Rate	Resolution	Sensor Active Area
165 fps	816 x 624 pixels	7.3mm x 5.6mm
306 fps	512 x 512 pixels	4.6mm x 4.6mm
1,015 fps	256 x 256 pixels	2.3mm x 2.3mm
1,295 fps	192 x 192 pixels	1.7mm x 1.7mm
1,572 fps	128 x 128 pixels	1.2mm x 1.2mm

4. Simple Setup by Just Connecting — Automated Synchronization Control and Stimulus Pattern Creation

By simply connecting each device to the ESTM10A, which acts as the command center, camera acquisition, light source illumination, pacing, and ECG recording can be automatically synchronized.

This reduces the stress caused by complex wiring and settings and supports stable, error-free experiments.

The high-speed camera settings and data acquisition are handled by BV Workbench.

• Camera control and acquisition software (BV Workbench)

Synchronization of camera acquisition, light source illumination, and stimulation is handled by the control software for the ESTM10A.

• Software for synchronization control, electrical stimulation and analog recording (ESTM10A)

5. Pacing Is Also Possible — Pulse Output Function

The multifunction electrical stimulator ESTM10A has an output terminal with a built-in isolator. By connecting stimulating electrodes, electrical stimulation of animal samples is also possible.

It also provides modes for creating stimulus patterns such as S1-S2 and S1-S2-S3-S4, which are used when applying electrical stimulation to the heart.

6. ECG Recording Synchronized with Image Data Is Also Possible — Analog Recording Function

The multifunction electrical stimulator ESTM10A is equipped with 2 channels of analog input. By connecting an ECG amplifier (sold separately), ECG recording synchronized with image data is also possible.

7. High-Precision Data Analysis and Map Creation with Simple Operation — Dedicated Data Analysis Software

Data analysis uses BV Workbench, the data analysis software bundled with the higher-end models. You can easily output high-resolution map images for use in scientific papers and presentations.

8. Adds No Noise to Biological Signals — Stable LED Light Source with Minimal Fluctuation

The system adopts "LEX5," an LED light source dedicated to high-speed imaging. A feedback mechanism that constantly monitors brightness and suppresses flickering prevents unnecessary noise from entering the data. Because brightness fluctuation is kept below 0.3% even during 30 seconds of illumination, even subtle signal changes can be clearly distinguished.

• High-powered, high-stability LED light source LEX5

9. Add Functions Later as Your Research Progresses — Flexible Customization Supported

You can upgrade each part later according to changes in research direction or budget. Because the system can evolve flexibly to match future needs, you can use the equipment you have introduced for a long time without waste.

Example of Request	Details	Example of Change
Improved S/N ratio	Change to a higher-end camera	Change to MiCAM03-N256
Multi-wavelength measurement	Expansion to a system for simultaneous measurement of membrane potential and calcium transient	Add a fluorescence splitter and fluorescence filters Add a light source or change to LEX9
Change of target animal	Change to a lens suitable for guinea pigs, rabbits, etc.	Change to a low-magnification lens
Easier focus adjustment	Add a fine focus adjustment function	Change to a motorized focus drive

BV-XB2 System
Name	Optical Mapping System BV-XB2
Model	BV-XB2
Standard System Configuration	· Camera head Alvium 1800 U-052m · Data analysis software BV Workbench · Camera lens + simple stand · Multifunction electrical stimulator ESTM10A · High-powered, high-stability LED light source LEX5-G · 1 bifurcated light guide + 2 condensing lenses · 2 flexible arms for holding light guides · Computer · LCD monitor · Cables
Camera Head
Image Sensor	Sony CMOS IMX426 (1/1.7 inch)
Resolution (H x V)	816 x 624
Active Image Area (H x V)	7.3mm x 5.6mm
Pixel Size (H x V)	9.0µm x 9.0µm
Shutter Mode	Global Shutter
Spatial Resolution and Maximum Frame Rate	165fps@816x624 pixels 306fps@512x512 pixels 1,015fps@256x256 pixels 1,295fps@192x192 pixels 1,572fps@128x128 pixels
Quantum Efficiency	73% (@529nm)
Dark Noise	21.8 e-
Saturation Capacity	100,000 e-
Pixel Bit Depth	12bit
Interface	USB3 Vision
Lens Mount	C-mount
Dimensions (L x W x H) / Weight	38mm x 29mm x 29mm / 60g
Synchronization Control / Electrical Stimulator ESTM10A
Input Terminals	Analog (2ch) / Bit (2ch) / Trigger (1ch)
Output Terminals	Pulse (4ch) / LED (3ch) / Isolated (1ch) / Analog (2ch)
I/O Terminals	V-sync (1ch) / Shutter (1ch)
Digital Input	Input voltage: 0V/5V
Digital Output	Output voltage: 0V/5V, Output current: 5mA
Analog Input	Input voltage: 10V
Analog Output	Output current: 5mA
Isolator Output	Output voltage: ±25V
Analog Output	Output voltage: ±5V
Dimensions / Weight	245mm (W) x 90mm (H) x 180mm (D) / 1.3kg
LED Light Source LEX5
Center Wavelength	530nm
Number of Built-in LEDs	1
Mountable Filter	25mm fluorescence filter x 1
Brightness Control (Manual)	Coarse 0–120% / Fine 0–10%
Brightness Control (Software)	0–120%
Illumination Control	Manual switch External signal input (> 1.4V) Software
Illumination Mode	CC (constant current) mode OFB (optical feedback) mode
Brightness Drift during 30 s of Illumination	< ± approx. 0.3%
Optical Output Noise	< ± approx. 0.01%
Input Terminals	BNC terminal (for illumination control) x 1 USB mini-B terminal (for software control) x 1
Acquisition / Analysis Software
Supported OS	Windows 11
Recommended PC Specifications	Minimum · Any Intel or AMD x86-64 processor · 8 GB of RAM · 400 MB of disk space for installation Recommended · Intel or AMD x86-64 processor with 4 logical cores and the AVX2 instruction set · 32 GB of RAM · NVMe solid-state drive · NVIDIA GPU
Optics
Objective Lens	24mm/1.4 camera lens
Lens Mount	C-mount
Stand	Basic stand
Focus Adjustment	Manual coarse dial
Light Source Light Guide	Bifurcated light guide + 2 condenser lenses
Light Guide Holding Mechanism	2 flexible arms
Illumination Method	Oblique illumination
Supported Fluorescence Filter	50mm fluorescence filter

* Specifications and appearance are subject to change without prior notice due to continuous improvements.
* This product is for research use only. * This product is made in Japan.