MiCAM05-N256

Large Sensor and Large Pixel

Active Area size

17.6mm x 17.6mm

Pixel size

69μm x 69μm

Applications such as membrane potential imaging require both very high speed, and the ability to detect small changes in fluorescence, such as 0.5-1%. In order to optimize S/N ratios, it is important to increase the amount of light captured by one pixel. It is easy to collect light and capture small changes using a sensor having large pixels.

High Spatial Resolution, High Frame Rates, and High S/N Ratios

Number of Pixels

256 x 256 pixels

Maximum Frame Rate

1,818 fps (at 256x256 pixels)

Dynamic Range

71dB

The overall performance has been improved in a balanced manner. This includes maximum frame rate, spatial resolution, and S/N ratios, all of which are important factors for high-speed membrane potential imaging.

Wide Dynamic Range Mode or High Sensitivity Mode by Adjustable Well Depth

Wide Dynamic Range Mode

3,000,000 electrons

High Sensitivity Mode

   600,000 electrons

It is now possible to easily switch the full well capacity of the camera in the acquisition software. Two modes are available: wide dynamic range mode (D-mode), and high sensitivity mode (S-mode). D-mode is used for tissue samples with high fluorescence light intensity, and S-mode is used for cultured cell monolayers, or preps with low fluorescence light intensity. This adjustable well depth makes it possible to image a variety of biological samples using a single camera.

ROI Readout for Higher Frame Rates

Preset partial readout setting and multiple ROI setting can be selected, and as a result it is possible to achieve higher frame rates. For example, ultra high frame rates such 20,000 fps@32x32 pixels is possible.

• All pixels readout (256x256 pixels)
• Multiple ROI readout (32x32x9 pixels)

Direct Data Saving and Long-Term Data Acquisition with USB3.0 High-Speed Data Transfer

New USB3.0 interface allows for significantly faster data transfer from processor to PC. It is now possible to save data directly to HDD or SSD, and long-term recording of several minutes up to a few hours can be achieved, regardless of the RAM capacity (Note that sampling rate, number of pixels, number of cameras used, and PC specifications, will affect the total recording time).

Disk Capacity	Pixels	Frame Rate (fps)	Number of recordable frames	Recordable time (min)
SSD 1TB	256x256	500	6,840,000	228.0
		1,000	6,840,000	114.0
		1,818	6,840,000	62.7
	128x128	500	27,360,000	912.0
		1,000	27,360,000	456.0
		5,263	27,360,000	86.6

(*Approximate number of recordable frames and recordable time shown above are averaged values that were measured using the PC configuration which is included with MiCAM05. There is no guarantee that the results will be the same in all circumstances.)

Up to four cameras can easily be connected and used in a completely synchronized multi-camera system

Up to two camera interface boards can be connected to the MiCAM05 processor. Each interface board is equipped with two camera ports, therefore up to four of the same type of camera head can be connected at any time. This allows for simultaneous imaging of multiple fluorescent wavelengths, as well as three-dimensional imaging from different angles.

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Continuous output of light intensity is available as a standard function

The light intensity monitor/continuous output function, available as an optional component of MiCAM Ultima (DAC/ADC expanding unit), is available as standard equipment with MiCAM05. This function is designed to output the light intensity changes of up to eight user selected pixels via eight BNC connections. Therefore, waveforms of fluorescence change can be monitored and output before, during, and after data acquisition. It is also possible to monitor the change in light intensity in real time, using optional software.

• Light intensity outputs from eight BNC connections
• Wave monitor on software

Synchronous Lighting Control of LED Light Source for Multi-Wavelength Excitation Imaging (Optional)

In the case of imaging using multiple fluorescent probes with different excitation wavelengths or imaging using a two-wavelength excitation/single-wavelength fluorescent probe, it is necessary to synchronize the frame timing of the camera and the lighting timing of the light source.

This function is to output the lighting signal synchronized with each frame timing from MiCAM05 to multiple LED light sources. This makes it possible to easily synchronize the frame acquisition with MiCAM05 and LED lighting.

There are up to 4 channels of output. Preset patterns, in which signals are output alternately from each channel, are available. There is no need to set the lighting pattern using a commercially available pulse generator.

Moreover, since the pulse delay time and pulse width can be specified by software, it is possible to make detailed settings in accordance with the rise and fall times of the LED used.

• Optional Unit
• Two-wavelength excitation mode Four-wavelength excitation mode

Various I/O Connectors, and Improved Compatibility with External Devices

MiCAM05 has multiple input and output connections available, in comparison to previous MiCAM systems. These universally standard I/O connections can be synchronized with image acquisition and external devices. An additional slot has been added to MiCAM05 so that customization is also available, depending on the research application.

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Automatic Light Control and Adjustment of Light Intensity

In addition to the “Shutter” output terminal for controlling the timing of illumination in synchronization with image acquisition, there is a “LightCtl” signal output terminal for automatically adjusting the intensity of the excitation light. In this way, light intensity or saturation percentage of the image captured by the camera, can be adjusted using the software. As the background light intensity becomes similar throughout an experiment and even for different biological preps, the experimental repeatability can be improved.

Turnkey System Ready for Immediate Use

The system consists of hardware/software specifically designed for high-speed fluorescence imaging, and the PC and monitor are included as standard equipment. Before use, calibration/setting/tuning is not necessary. The hardware connection is straightforward between components using cables, making it possible to acquire data immediately after setup.

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Pre-Post Trigger Mode

MiCAM05 can record activity which occurs before and after a trigger signal is generated. When an external trigger occurs, such as a spontaneous biological signal, or a trigger pulse from an external stimulator, a specified amount of "pre-trigger" data can be saved. The "post-trigger" data is also collected in the same recording. As a result, both pre-trigger data and post-trigger data can be saved within one acquisition period.

• Pre-Post Trigger Mode (75% Pre-Trigger)

Time Lapse Imaging

MiCAM05 can capture phenomenon that changes in a few minutes up to several hours, intermittently. Because illumination light is turned on only at the time of image acquisition, phototoxic effects to sample is minimized, and a stable baseline can be acquired.

• Example of Time-lapse imaging

High Resolution Camera Control Function (Optional)

It is possible to control a high resolution camera having 1920x1200 pixels (BV-XB1 camera). High resolution imaging can be applied to various applications, including detection of intracellular Ca 2+ dynamics, flavoprotein fluorescence, FRET imaging, and also imaging of animal behaviors. High resolution imaging with BV-XB1 can also be synchronized with MiCAM05 high-speed imaging.

• BV-XB1
• Example of flavoprotein imaging

Acquisition/Analysis Software (BV Workbench)

BV Workbench is the image acquisition software that controls MiCAM05-N256 optical mapping system. In addition to image acquisition and control of inputs and outputs of the imaging system, analysis functions including movie playback, wave display, filter functions, etc…are also included. BV Workbench has an intuitive user interface that is easily operated with the use of a mouse.

System
Name	High Speed Optical Mapping System MiCAM05-N256	High Speed Optical Mapping System MiCAM05-HR
Model	MC05-N256	MC05-HR
Standard System Configuration	Camera Head (1-4), Processor, PC, Monitor, Acquisition software, Analysis Software
Supported OS	Windows 7/10 64bit
Camera Head
Name	N256 camera	HR camera
Model	MC-N256CAM	02HRCM
Image Sensor	Original CMOS	Sony CCD
Active Pixels (H x V)	256 x 256	380 x 244
Active Image Area (H x V)	17.6mm x 17.6mm	6.4mm x 4.8mm
Pixels Size (H x V)	69μm x 69μm	16.8μm x 19.6μm
Shutter Mode	Global	Global
Maximum Frame Rate and Pixels (for 1 camera)	1,923fps (256x256px) 3,125fps (192x192px) 5,556fps (128x128px) 20,000fps (32x32px)	143fps (380x244px) 270fps (188x120px) 455fps (92x60px)
Quantum Efficiency	55%@550nm / 50%@600nm	75%@550nm / 50%@700nm
Well-Depth	3,000,000 e- / 600,000 e- (switching)	100,000e- (180x120px) / 12,000e- (180x120px, H-bin)
Dark Noise	500 e- / 130 e-	60e- (3.7ms,180x120px) / 20e- (3.7ms,180x120px,H-bin)
Lens mount	Original M42 mount (flange back:14.5mm) (Option: C or F mount)	C mount
Interface	Special Cable	Special Cable
Dimension (WxHxD)	80mm x 80mm x 50mm	62mm x 52mm x 46mm
Weight	474g	100g
Processor
Name	MiCAM05 processor
Model	MC05-PRC
Camera Interface	1 (up to 2)
Camera Port	2 (up to 4)
Slot for Optional Unit	1
Buffer Memory Inside Processor	4GB (up to 8GB)
Analog Inputs for Synchronous Recording	4ch
Output for Stimulation Pulse	2ch
Input for External Trigger Signal	1ch
Output for Lighting Control	1ch
Input/Output for Frame Timing	1ch
Output for Real Time Light Intensity Monitor	8ch
Output for Light Intensity Control	2ch
Current Output	2ch
Output for Acquisition Status	1ch
Output for Trigger Wait Status	1ch
Digital I/O port	1 (25 pins)
Trigger Mode	Software / TTL External Input / Analog Input / Usage of Optional Unit
Data Transfer Mode	USB3.0 (for basic functions) / USB2.0 (for Real Time Light Intensity Monitor)
Recording Mode	Temporary storage to PC RAM / Direct data saving to PC drive (SSD/HDD)
Maximum Recordable Frame Number	Dependent on camera used and image acquisition settings
Power Supply	AC100V-230V、50Hz/60Hz 150W
Fuse	T2A L 250V
Dimension	430mm (W) x 350mm (D) x 75mm (H)
Weight	6.2kg
Default/Optional Function
Single Trial Acquisition	Default
Long Time Acquisition (direct saving to disk)	Default
Averaging Acquisition	Default
Multiple ROI Readout for Higher Frame Rate	Default
Time-Lapse Acquisition	Default
High Resolution Camera Control	Option (sold separately)
Acquisition software	Default
Analysis software	Default
SDK (for Visual C# .NET and MatLab)	Option (sold separately)

* Specifications and appearance are subject to change without prior notice due to continuous improvements.
* This product is for research use only. * This product is made in Japan.

Selectable Number of Pixels (HxV)		Maximum Frame Rate (fps)
		NR Mode OFF	NR Mode ON 2 Times Averaging	NR Mode ON 4 Times Averaging
256x256	Full Resolution	1,818	1,923	1,031
192x192	Square ROI	2,941	3,125	1,695
128x128		5,263	5,556	3,333
64x64		12,500	12,500	8,333
256x128	Horizontally Long Rectangle ROI	3,333	3,448	1,887
256x64		5,882	5,555	3,226
256x32		9,091	8,333	5,000
128x256	Vertically Long Rectangle ROI	3,030	3,333	1,852
64x256		4,348	5,000	3,125
32x256		5,556	7,143	4,762
32x32	16 blocks	2,381	2,632	1,724
	15 blocks	2,500	2,778	1,818
	14 blocks	2,703	2,941	1,961
	13 blocks	2,857	3,125	2,083
	12 blocks	3,125	3,448	2,273
	11 blocks	3,333	3,704	2,439
	10 blocks	3,704	4,000	2,703
	9 blocks	4,000	4,348	2,941
	8 blocks	4,545	5,000	3,226
	7 blocks	5,000	5,556	3,704
	6 blocks	5,556	6,250	4,167
	5 blocks	6,667	7,143	5,000
	4 blocks	7,692	8,333	5,882
	3 blocks	10,000	11,111	7,692
	2 blocks	12,500	14,286	10,000
	1 blocks	20,000	20,000	16,667

Selectable Number of Pixels (HxV)		Maximum Frame Rate (fps)
		NR Mode OFF	NR Mode ON 2 Times Averaging	NR Mode ON 4 Times Averaging
256x256	Full Resolution	1,087	1,064	1,031
192x192	Square ROI	1,818	1,754	1,695
128x128		3,704	3,571	3,333
64x64		9,091	8,333	8,333
256x128	Horizontally Long Rectangle ROI	2,041	1,961	1,887
256x64		3,571	3,226	3,125
256x32		5,882	5,000	5,000
128x256	Vertically Long Rectangle ROI	2,000	1,961	1,852
64x256		3,571	3,448	3,125
32x256		5,556	5,556	4,762
32x32	16 blocks	1,887	1,613	1,613
	15 blocks	2,041	1,724	1,724
	14 blocks	2,174	1,852	1,852
	13 blocks	2,326	1,961	1,961
	12 blocks	2,500	2,128	2,128
	11 blocks	2,703	2,326	2,326
	10 blocks	2,941	2,500	2,500
	9 blocks	3,226	2,778	2,778
	8 blocks	3,571	3,125	3,125
	7 blocks	4,000	3,448	3,448
	6 blocks	4,762	4,000	4,000
	5 blocks	5,556	4,762	4,762
	4 blocks	6,667	5,556	5,556
	3 blocks	8,333	7,143	7,143
	2 blocks	11,111	10,000	10,000
	1 blocks	16,667	16,667	16,667

System
Name	MiCAM05-Ultima	MiCAM05-D225
Model	MC05-UL	MC05-D225
System
Name	Ultima camera	D225 camera
Model	CAM-ULT	D225
Image Sensor	Original CMOS	Original CMOS
Active Pixels (HxV)	100x100 (fixed)	240x256 (fixed)
Active Image Area (HxV)	10.0mmx10.0mm	7.2mmx7.7mm
Pixel Size (HxV)	100μmx100μm	30μmx30μm
Shutter Mode	Rolling Shutter	Global Shutter
Maximum Frame Rate (for 1 camera)	10,000fps	1,000fps
Well-Depth	1,500,000 e-	500,000 e-
Lens Mount	C mount	C mount
Dimension (WxHxD)	80mmx65mmx50mm	75mmx65mmx50mm
Weight/th>	270g	246g
Processor
(Same as MiCAM05-N256)