The DLFLSP2R is a fluorescent beam splitter that transmits two wavelengths of light simultaneously to two cameras.
- Fluorescence Beam Spliter
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DLFLSP2R
Main Features
- Split fluorescent light into two different wavelengths to two separate cameras
- Easily overlay images of two different wavelengths
- Can be used as an epi-fluorescence filter unit for single wavelength fluorescence imaging.
Applications
- Simultaneous imaging of biological preparations stained with both a calcium indicator and a voltage-sensitive dye
- Fluorescent ratiometric imaging using calcium indicators or voltage-sensitive dyes
Features
The DLFLSP2R can be with or without Brainvision's tandem-lens macroscope
(see Fig.1 and 2). Both configurations adopt the tandem-lens style which
includes Leica PLAN APO lenses, wide optical pathway, and large
custom-made fluorescence filter cubes. These systems are ideal for
detecting very small signals which are typical in membrane voltage
imaging.
The DLFLSP2R splits fluorescent light emitted from a biological
preparation into two different wavelengths to two separate cameras, with
the use of a large, custom-made dichroic mirror. The dichroic mirror
uses a kinetic mount with straightforward shift and tilt adjustment
knobs which allows users to easily overlay images of two different
wavelengths.
An excitation light source light guide can be connected to the side of
the DLFLSP2R with the use of an adaptor (fig.3). In this way, the
DLFLSP2R can be used as an epi-fluorescence filter unit for single
wavelength fluorescence imaging.
- Fig.1 (left) Setting for imaging two wavelengths simultaneously by combining two DLFLSP2Rs (uses epi-illumination)
- Fig.2 (center) Setting for imaging two wavelengths simultaneously (uses side illumination)
- Fig.3 (right) Setting for imaging one wavelength by inserting a light guide (uses epi-illumination)
How to Overlay Dual Camera Images
Examples
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Epi-illumination/dual wavelength imaging (upright)
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Epi-illumination/dual wavelength imaging (45 degrees)
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Epi-illumination/dual wavelength imaging (90 degrees)
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Dual DLFLSP2R (can use both Epi and Side Illumination)
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Dual DLFLSP2R (can use both Epi and Side Illumination)
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Combination with THT component (can use both Epi and Side
Illumination)
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DLFLSP2R without THT component (uses only side
illumination)
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Combination with THT-sideways (can use both Epi and Side
Illumination)
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DLFLSP2R without THT component (uses only side illumination)
Specifications
| Filter Size |
Dichroic Mirror (x1): 50mmx70mm Emission Filter: 50mm diameter |
|---|---|
| Lenses |
Leica Microsystems PLAN APO lenses (Attached to top, bottom and left side.) |
| Positioning mechanism | Two Kinematic Mount Adjustment Knobs |
| Applicable Microscope | Brainvision's tandem-lens macroscope (THT series) |
Related Product
- Fluorescence Mesoscope
- THT Series
The THT is a fluorescence widefield mesoscope developed for detecting low light fluorescence that is emitted from biological sample at a low magnicifation. This mesoscope has a very simple optical path combining two large-diameter objective lenses and a custom-made large fluorescent filter.