List of analysis function
Image analysis
| Item | Description |
|---|---|
| Spatio-temporal analysis | Display time-distance map on a straight line or polyline on image |
| Time domain analysis | Create a map of all peaks within a specified area |
| - Activation time map | Calculate activation time and create an activation time map |
| - Rise time map | Calculate rise time and create rise time map |
| - Peak time map | Calculate peak time and create peak time map |
| - Peak amplitude map | Calculate peak amplitude and create peak amplitude map |
| - Repolarization time map | Calculate repolarization time and create repolarization time map |
| - Peak to repolarization time map | Calculate time from peak time to repolarization time and create a “Peak to repolarization time” map |
| - APDxx map | Calculate AP duration and create APDxx map |
| - Decay τ (tau) map | Calculate time constant (τ) in decay time of AP and create Decay τ" map |
| - Upstroke velocity map | Calculate maximum upstroke velocity and create upstroke velocity map |
| - Diastolic interval map | Calculate diastolic time and create diastolic interval map |
| - Peak interval map | Calculate time between peaks and create a peak interval map |
| - Alternans map | Create amplitude alternans map |
| - APDxx alternans map | Create APD alternans map |
| Conduction velocity analysis | Display and image output of conduction velocity map, CSV output |
| Leading region map | Display of action potential (calcium transient) initiation site |
| Dominant frequency analysis | Dominant frequency map display, image output, CSV output |
| Phase analysis | Phase map display and image output, CSV output Display of Phase Singularity Display of PS (phase singularity) trajectory |
| Wave map | Divide image evenly and display average waveforms of each area on the image |
Waveform analysis
| Item | Description |
|---|---|
| Firing rate plot | Show firing rate per time |
| Frequency spectrum | Display frequency analysis results using Fourier Transform |
| Spectrogram | Display time-frequency analysis results using Continuous Wavelet Transform |
| Phase plot | Display phase information using Hilbert Transform |
| Waveform analysis | Displays average and standard deviation of Peak amplitude / Rise time / xx% decay time / Decay constant / APDxx / Diastolic interval / Peak interval / Alternans / Upstroke velocity. BPM is also displayed. |
Image filter
| Item | Description |
|---|---|
| Undo filters | Undo the last filter |
| Filter toolbox | Run multiple filters at once |
| - 2D Convolution filter | Noise removal by Mean filter or Gaussian filter |
| - Median filter | Noise removal by median filter filter |
| - Drift removal | Correction for photobleaching curve |
| - Smoothing filter | Noise removal by Moving average or Savitzky-Golay |
| - Wavelet filter | Wavelet filter |
| - Windowed-Sinc filter | Low-pass or High-pass or Band-pass or Band-stop |
| - Invert signals | Invert image and waveform polarity |
| - Normalize amplitude | Compensate for differences in amplitude of light intensity between pixels, and calculate so that light intensity values of all pixels have the same amplitude (0 to 65,535). |
| Illumination correction | Brightness correction / illumination non-uniformity correction |
| Lens correction | Correction for lens distortion |
Other calculations
| Item | Description |
|---|---|
| Alignment | Enlarge / reduce / rotate / move two images and overlay them |
| Binning | Combine multiple pixels into one pixel (addition or average) |
| Average datasets | Averaging data from multiple datasets offline |
| Crop | Specify an area and crop image |
| Deinterleave frames | Extract frames of data acquired by multi-wavelength excitation imaging such as GCaMP and Fura-2 and split them into multiple data |
| Frame operation | Add / subtract / multiply / divide between two data in the same data set |
| Create subset | Specify time range and open as separate data |
Loading/adding data
| Item | Description |
|---|---|
| Loading/adding data | |
| Loading an image dataset | Support file extension “bvx”/”json” |
| Adding image data to an image dataset | Support file extension “raw”/ “tif” / “gsd” |
| Loading and analyzing multiple datasets | Managed on tab page |
| Displaying data information | View acquisition settings, comments, filter history, etc. |
Image display
| Item | Description |
|---|---|
| Image drawing mode | F : absolute value / F0 : reference value / ⊿F: difference value / ⊿F/F0 : rate of change (%) / F(x)-F(x+1) / F(x)/F(x+1) |
| Setting analysis area using a mask | Specify threshold by light intensity value and set analysis area |
| Draw a straight line / polyline / rectangle / polygon / circle and set analysis area | Click on an image to set analysis area |
| Zoom in / Zoom out | Operate by scrolling mouse wheel |
| Change color map | New color map creation, import and export are also possible |
| Change reference image | Minimum value / average value of specified range / display frame / |
| other image data (tiff, raw) | |
| Change background image | Display frame / external image file (bmp, jpg, png, tif) |
| Display light intensity histogram | |
| Displaying a scale bar and setting scale | Display can be turned on or off |
| Display of XY coordinates | Mouse over image |
| Numeric data display of coordinates | Mouse over image |
| Time and frame number display |
Video display
| Item | Description |
|---|---|
| Play/Stop video | Video playback speed can be changed |
| Frame forward/Frame backward | Frame number can also be specified |
Waveform display
| Item | Description |
|---|---|
| Click on image to display signal at that point as a waveform | Any number of points can be specified. Can be moved by mouse dragging. |
| Specify a rectangle / polygon /circle on image and display average waveform of that area | Any number of points can be specified. Can be moved by mouse dragging. |
| Simultaneous display of analog waveforms such as ECG | Display can be turned on or off |
| Move waveform display point by dragging point on image with mouse. | Waveform display changes accordingly |
| Manually specify coordinates of waveform display points | |
| Polarity inversion | Can be specified individually |
| Change display color | Can be specified individually |
| Resize display point (averaging) | 1x1, 3x3, 5x5, 7x7, 9x9, 11x11, 13x13, 15x15 (pixels) |
| Move display frame by dragging mouse on waveform | Image display is changed |
| Specifying a time range | Can be specified by mouse drag or numerical input |
| Change in value calculation method | F : absolute value / ⊿F : differential value / |
| dF/dt : first derivative of absolute value / | |
| dF2/dt2 : second derivative of absolute value / | |
| d(⊿F/F0)/dt : first derivative of change rate | |
| Change units of vertical axis | % or raw value |
| Vertical gain adjustment | |
| Zoom in/out on horizontal axis (time axis) | Operate by scrolling mouse wheel |
Data output
| Item | Description |
|---|---|
| Image (numeric) | tiff / csv |
| Image | png / svg |
| Waveform (numeric) | csv |
| Image including waveforms | png / svg |
| Video | avi |
General settings
| Item | Description |
|---|---|
| Specify number of times to "undo" | Specify maximum number of times that you can undo |
| Use of GPU ON/OFF | It is possible to speed up the following calculation processing using GPU. Drift removal / Phase analysis / Time-Frequency analysis / Frequency domain analysis |
| Custom color map | Add / delete / import / export color map information |
| Importing software licenses / Request for a new software license | Issue a software license based on hardware ID unique to PC in use |