Sample Data
Calcium Imaging of Cultured Cardiomyocyte Sheet
- Sample
- Cultured cardiomyocyte sheet
- Fluorescence Dye
- Calcium Dye (Cal-520)
- Imaging System
- MiCAM05-C35IR
- Pixels
- 636x360
- Frame Rate
- 200fps (5msec/frame)
- Provided by
- Dr. Hiroko Izumi-Nakaseko, Department of Pharmacology, Faculty of Medicine, Toho University
High-Speed Calcium Imaging of Isolated Mouse Atrial Cardiomyocytes
- Sample
- Freshly isolated mouse atrial cardiomyocytes. Cells were plated on glass cover slips coated with laminin and used on the same day as isolation.
- Method
- Cells were paced by electrical field applied through a perfusion solution. Imaging was performed on Nikon DIAPHOT 300 microscope with 20X lens and SPECTRA-X Lumencor light source.
- Fluorescence Dye
- Calcium sensitive dye (Fluo-4 AM)
- Imaging System
- MiCAM05-ULTIMA
- Pixels
- 100x100
- Frame Rate
- 200fps (5.0msec/frame)
- Provided by
- Dr. Roman Medvedev and Dr. Alexey Glukhov, Department of Medicine, University of Wisconsin-Madison
Monolayer of neonatal mouse ventricular myocyte stained with voltage sensitive dye
- Sample
- Monolayer of neonatal mouse ventricular myocyte. Sample was scratched with a needle tip at three locations (visible on the fluorescence pictures) to induce conduction blocks and meandering of the wavefront.
- Method
- Sample is paced locally at different frequencies using a glass microelectrode positioned at the edge of the sample
- Fluorescence Dye
- Voltage sensitive dye (Di-8-Anepps)
- Imaging System
- MiCAM03-N256
- Pixels
- 256x256
- Frame Rate
- 1,000fps (1.0msec/frame)
- Provided by
- Dr. Jan Kucera and Dr. Ange Maguy, Department of Physiology, University of Bern
Calcium Imaging of Cultured Cardiomyocytes (Fluo-4)
The preparation was cultured rat cardiomyocytes stained with Fluo-4 and activity was recorded using MiCAM01 (old model) in 2002. The current model, MiCAM02 can significantly improve the data in terms of spatial resolution, sensitivity and S/N ratio.
Voltage-Sensitive Dye Imaging of Cultured Neonatal Rat Ventricular Cardiomyocytes
Sustained spiral wave reentry in a 3 day old monolayer disc of cultured neonatal rat ventricular cardiomyocytes (frequency ~ 5 Hz; diameter of the preparation = 8 mm). The preparation was stained with the voltage sensitive dye di-8-ANEPPS and activity was recorded at 1,000 frames/second using a custom-made tandem lens macroscope and an Ultima-L camera system.
- Provided by
- Dr.Stephan Rohr, University of Bern
Product suitable for this kind of application
- High Speed Imaging System
- MiCAM03-N256
MiCAM03-N256 is a high-speed imaging system that captures and visualizes small changes in fluorescence intensity from biological samples stained with fluorescent probes, such as voltage sensitive dyes and calcium dyes.
- Spatial Resolution :
- 128x128 - 256x256 pixles
(32x32 pixels - 256x256 pixles with option) - Maximum Frame Rate :
- 1,000fps
(20,000 fps available with option) - Up to 2 camera heads can be used with completely synchronization.
- Ultra-High Sensitivity, Wide-Field Imaging System
- MiCAM03-C35IR
MiCAM03-C35IR is an imaging system equipped with a large 35mm full-size CMOS sensor, which has a spatial resolution of 2,160x1,280 pixels.
This model uses an ultra-high sensitive CMOS sensor which has large pixels, uses low noise technology, and has > 80% quantum efficiency. This makes it possible to image dim fluorescent samples at wide fields of view.
- Wide Field of View :
- 35mm full-size CMOS sensor
(47.7 mm diagonal) - Spatial Resolution :
- 2,160x1,280 pixels
- Maximum Frame Rate :
- 277.8 fps at 1,212x360 pixels