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THT-microscope

Overview

The THT-microscope is a tandem-lens fluorescence microscope developed for membrane potential imaging and calcium imaging.

Features of the THT-microscope include its simple yet flexible design, large light path for maximum throughput, and its large custom-made fluorescence filter sets. This allows for the detection of very small signals which are common in membrane voltage imaging.

Description

The THT-microscope is necessary to detect small changes in fluorescence light intensity in optical imaging for biological samples stained with voltage-sensitive dye. In addition to utilizing a fast and highly sensitive imaging system for membrane voltage imaging, it is equally important for researchers to use a fluorescence microscope which possesses a high light throughput. The THT-microscope is a custom-made tandem-lens fluorescence microscope developed especially for fluorescence imaging. Leica Microsystems's PLAN APO lenses can image low magnifications with extremely high N.A values. The THT-microscope can also be attached to other cameras using its standard C-mount. The THT-microscope combined with the HL-151 halogen light system provides an ideal optical configuration for fluorescence imaging.

Features

Simple Design Specific for Fluorescence Imaging

The THT-microscope is a fluorescence microscope developed for detecting low light fluorescence by capturing an optimal amount of light photons which are emitted from the biological sample. This microscope is a tandem-lens system which combines two Leica lenses with large fluorescence filters in order to maximize the amount of emitted light.


Various Types of Microscope Stands

Users can select among various microscope stand and stage configurations according to their specific research purposes.









Various Types of Objective Lenses

The THT-microscope includes Leica Microsystems's high NA value PLAN APO lenses. The combination of low-magnification lenses makes it possible to image relatively large areas in slice and in-vivo samples. It is also possible to image a more localized area by using higher magnification lenses.


Inverted Tandem-Lens Fluorescence Microscope


An inverted tandem-lens macro fluorescence microscope was developed for voltage sensitive dye imaging and calcium imaging. This inverted tandem-lens fluorescence microscope will give users flexibility to manipulate the biological preparation and place electrodes. The system can be used with the upright tandem-lens fluorescence microscope to simultaneously image different areas of a small sample. The sample stage and base of this inverted tandem-lens macro fluorescence microscope are optional parts. These parts can be customized based on the sample or purpose.


Complete System

Complete system includes MiCAM imaging system, PC, monitor, software for data acquisition/analysis, THT macroscope, light source and electrical stimulator.


Applications

  • Optical Mapping of Membrane Potential or Calcium
  • Simultaneous Optical Mapping of Membrane Potential and Calcium
  • Optical Recording of Cultured Cells
  • In Vivo Optical Recording of Small Animals
  • Using Inverted Microscope together with Upright Microscope

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