Voltage-sensitive dye imaging of in-vivo mouse with MiCAM ULTIMA.
This data was obtained from a 2 month-old mouse that was under urethane anaesthesia (1.75 g/kg). A heating pad was used to maintain the measured body temperature of the mouse at 37°C. Two electrodes were inserted under the skin of the forearms to receive the electrocardiogram signal which was used to trigger the MiCAM ULTIMA acquisition.

Animal

Mouse

Sample

In Vivo (Somatosensory Barrel Cortex)

Method

Piezo Whisker Flick Stimulation

Dye

RH1691

Sampling Rate

2.0ms/frame

Averaging

10 times

Provided by

Dr.Isabelle Ferezou and Dr.Carl C.H. Petersen
Brain Mind Institute, EPFL, Switzerland

Reference

Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving Mice.
Neuron. 2006 May 18;50(4):617-29.